Zeiss Axiovert 200 Microscope
Manufacturer:
Model:
Axiovert 200
Date:
2001
Category:
Group:
Description:
Inverted Microscopes

Information

The Axiovert 200 microscopes are universally applicable inverted microscopes and are mainly used for the examination of cell and tissue cultures and of sediments in culture flasks, Petri dishes, microtiter plates, etc. in transmitted and reflected light. The Axiovert 200 microscopes permit the performance of the transmitted-light techniques brightfield, phase contrast, differential interference contrast and VAREL contrast, and the epi-fluorescence technique. The microscopes Axiovert 200 and Axiovert 200 M are the basis for scientific microscopic work on living cells. − The sturdy stand provides attachment possibilities for various tools (micromanipulation), different light sources, temperature control devices, etc. − Ample space is available for the specimens and the relevant handling systems on account of the inverted design, the LD illumination system of the Microscope and the use of fixed stages. This permits the performance of experiments which would not be possible with upright microscopes. − The design allows the easy attachment of cameras, lasers, specific stages, etc. Instrument description and main features The Axiovert 200 / Axiovert 200 M is available either as manual or as motorized version. The accessory components are part of a modular system. For documentation purposes, the Axiovert 200 / Axiovert 200 M can be equipped with the maximum of five camera / TV ports in accordance with the customer's requests. Adaptation possibilities are provided for heating stages, incubators and micromanipulators. Major instrument features: − ICS optics for image creation − high thermal and mechanical stability − high flexibility in documentation − improved ergonomic design − LCD display of instrument parameters − 23 mm field of view − Light Manager − modular design for optimum adaptation to the relevant application − 6-position nosepiece, coded − 5-position reflector turret, manual: can be changed on both sides; motorized: can be changed from the right − 5-position or 6-position condenser turret − 3-position Optovar turret − changeable aperture diaphragm and luminous-field diaphragm sliders in reflected light − fluorescence shutter − illuminators: HAL 100 W, HBO 50, N HBO 103, N XBO 75 − Axiovert 200 M, all the major Microscope functions are motorized.

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User Manual
Manual type:
User Manual
Pages:
120
Size:
5.21 Mbytes (5466843 Bytes)
Language:
english
Revision:
Manual ID:
B 40-080 e
Date:
30 March 2001
Quality:
Electronic document, no scan, very well readable.
Upload date:
20 February 2018
MD5:
f73d2cbd2f2a9f90a504bf6d77d75c7d
Downloads:
783

Information

INTRODUCTION ... 0-2 Copyright ... 0-2 Contents ... 0-3 Notes on instrument safety ... 0-7 Notes on warranty ... 0-10 Overall view of the Axiovert 200 ... 0-11 Overall view of the Axiovert 200 M ... 0-11 Microscopy in transmitted-light brightfield in a few steps ... 0-12 CHAPTER1.1 1 INSTRUMENT DESCRIPTION ... 1-3 Name and intended application ... 1-3 Instrument description and main features ... 1-5 Microscope configurations and modules ... 1-6 Objectives ... 1-10 Eyepieces ... 1-12 Condensers ... 1-12 Specimen stages and mounting frames ... 1-13 Binocular tubes ... 1-13 Technical Data ... 1-14 CHAPTER2.1 START-UP ... 2-5 Unpacking and installation of the microscope ... 2-5 Attachment of binocular (photo) tube ... 2-6 Inserting the eyepieces and the centering telescope ... 2-6 Inserting the eyepiece reticle ... 2-7 Attachment of transmitted-light illumination ... 2-8 Attachment of carrier for transmitted-light illumination (100 W) ... 2-8 Attachment of carrier for transmitted-light illumination (30 W) ... 2-8 Screw in objectives ... 2-9 Attachment of Microscope stages ... 2-10 Attachment of mechanical stage 130x85 R/L and mounting frame for mechanical stage (K) ... 2-10 Attachment of scanning stage ... 2-10 Attachment of specimen stage 250x230, object guide and mounting frame for object guide (M) ... 2-11 Attachment of heating stage ... 2-12 Attachment of gliding stage Z ... 2-12 Attachment of condensers ... 2-13 Condensers for the Axiovert 200 ... 2-13 Condensers from the Axioplan 2 imaging / Axioskop 2 product line ... 2-13 Changing the DIC prism in the condenser turret ... 2-14 Reflector turret ... 2-15 Attachment of reflector turret ... 2-15 Equipment of reflector turret ... 2-15 Changing the filter set in the FL reflector module ... 2-16 Changing the beam splitter in the FL reflector module ... 2-16 Connection to the line ... 2-18 Interfaces of the Axiovert 200 M ... 2-19 Switch Microscope and ebq 100 dc power supply on and off ... 2-19 Equipotential bonding terminals ... 2-19 HAL 100 halogen illuminator ... 2-20 Change / attachment of the HAL 100 halogen lamp ... 2-20 Coarse alignment of halogen illuminator ... 2-21 Attachment of halogen illuminator ... 2-21 Fine alignment of halogen illuminator ... 2-22 Illuminator N HBO 103 ... 2-23 Attachment / change of the HBO 103 W/2 mercury pressure short-arc lamp ... 2-23 Coarse alignment of illuminator N HBO 103 ... 2-25 Attachment of illuminator N HBO 103 ... 2-26 Fine adjustment of illuminator N HBO 103 using the adjusting aid ... 2-27 Attachment of Aqua Stop ... 2-28 CHAPTER3.1 OPERATION ... 3-3 Axiovert 200 (manual) ... 3-4 Operation and function controls on the Axiovert 200 (manual) ... 3-4 Switching on and basic settings on the Axiovert 200 (manual) ... 3-14 Axiovert 200 M (motorized) ... 3-16 Operation and function controls on the Axiovert 200 (motorized) ... 3-16 Switching on and basic settings on the Axiovert 200 M (motorized) ... 3-23 Illumination and contrasting techniques ... 3-28 Setting of transmitted-light brightfield for KÖHLER illumination ... 3-28 Setting of transmitted-light phase contrast ... 3-33 Setting of differential interference contrast (DIC) in transmitted light ... 3-35 Setting of VAREL contrast in transmitted light ... 3-38 Setting of fluorescence contrast in reflected light ... 3-40 Documentation ... 3-43 Image orientation of camera ports ... 3-43 Photomicrography with SLR camera ... 3-45 Photomicrography using a digital camera and videomicroscopy ... 3-46 CHAPTER4.1 4 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ... 4-3 Care ... 4-3 Maintenance ... 4-4 Performing checks ... 4-4 Changing the fuses on the microscope ... 4-4 Changing the fuses on the ebq 100 dc power supply ... 4-5 Service ... 4-6 ANNEX ... A-1 List of abbreviations ... A-3 List of key words ... A-5 Certification in accordance with DIN ISO 9001 / EN 46001 EC Declaration of Conformity

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